Description Using a camera mounted above the microscope, experts adjust the lens and angle to capture the stain and transfer the image to a computer for processing.

Technical Details In this study used in-situ harvest method and trypsin/Wright stain procedure to prepare G-banding. Briefly, the amniotic cells were cultured in BIO-AMF medium for 6 to 8 days. Treating cells with colcemid for 30 min to arrest cells at metaphase. Swell cells with hypotonic solution and fix with methanol/acetic acid mixture. Fixed cells were treated with trypsin then stained with Wright stain solution . The karyotype was interpreted according to The International System for Human Cytogenomic Nomenclature (ISCN).

karyotype

A karyotype is an individual's complete set of chromosomes. The term also refers to a laboratory-produced image of a person's chromosomes isolated from an individual cell and arranged in numerical order. A karyotype may be used to look for abnormalities in chromosome number or structure.

Also a chromosomal karyotype is used to detect chromosome abnormalities and thus used to diagnose genetic diseases, some birth defects, and certain disorders of the blood or lymphatic system.

G-banding

G-banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. It is useful for the identification of structural abnormalities through the photographic representation of the entire chromosome complement. Down Syndrome Typically, a baby is born with 46 chromosomes. Babies with Down syndrome have an extra copy of one of these chromosomes, chromosome 21. A medical term for having an extra copy of a chromosome is 'trisomy.' Down syndrome is also referred to as Trisomy 21.

What is normal karyotype?

A normal human karyotype consists of 22 pairs of autosomes and two sex chromosomes. Note the similar size and striped (banding) pattern between each of the pairs. The autosomal chromosome pairs are numbered and arranged from largest to smallest.